Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrative approaches to determining Csl function.

T A Richmond1, C R Somerville

  • 1Carnegie Institution of Washington, Department of Plant Biology, Stanford, CA 94305, USA. todd@andrew2.stanford.edu

Plant Molecular Biology
|September 14, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drosophila P-element transcripts are incorrectly processed in tobacco.

Plant molecular biology·2013
Same author

Microarray-based capture of novel expressed cell type-specific transfrags (CoNECT) to annotate tissue-specific transcription in Drosophila melanogaster.

G3 (Bethesda, Md.)·2012
Same author

Diversity of conotoxin types from Conus californicus reflects a diversity of prey types and a novel evolutionary history.

Toxicon : official journal of the International Society on Toxinology·2010
Same author

A diverse family of novel peptide toxins from an unusual cone snail, Conus californicus.

The Journal of experimental biology·2010
Same author

Ultra-high resolution array painting facilitates breakpoint sequencing.

Journal of medical genetics·2006
Same author

Genetic dissection of plant cell-wall biosynthesis.

Biochemical Society transactions·2002
Same journal

Genetic basis of alkaloid divergence in the Solanaceae.

Plant molecular biology·2026
Same journal

Integrative approach to identify robust pattern recognition receptors in Eucalyptus grandis: novel candidates for disease resistance.

Plant molecular biology·2026
Same journal

Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.

Plant molecular biology·2026
Same journal

Coumarin metabolites in Ocimum: chemical diversity, biosynthetic pathways, and network pharmacology-based prediction of multi-target anticancer potential.

Plant molecular biology·2026
Same journal

Ethanol application enhances freezing stress tolerance in Arabidopsis and sugar beet.

Plant molecular biology·2026
Same journal

CeO<sub>2</sub> Nanozymes as redox regulators: Reprogramming of reactive oxygen species (ROS) signaling for plant climate resilience.

Plant molecular biology·2026
See all related articles

Researchers are investigating cellulose synthase-like (Csl) genes to identify enzymes involved in plant cell wall synthesis. While functions remain elusive, gene expression patterns suggest roles in primary cell wall formation.

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Biochemistry

Background:

  • The enzymes synthesizing structural plant cell wall polysaccharides, beyond cellulose synthase catalytic subunits (CesA), are largely unidentified.
  • The completion of the Arabidopsis genome and extensive sequence data offer opportunities to identify these crucial enzymes.
  • Arabidopsis possesses six gene families related to cellulose synthase, termed cellulose synthase-like (Csl) genes.

Discussion:

  • A multidisciplinary approach combining genomic, genetic, and biochemical data is employed to elucidate Csl gene functions.
  • Expressed sequence tag (EST) data indicate Csl genes are widespread across plant species, though some families, like CslG, appear absent in certain species (e.g., rice, maize).
  • Microarray data and reporter constructs are utilized to map the expression patterns of CesA and Csl genes in Arabidopsis.

Related Experiment Videos

Key Insights:

  • Mutations and insertion events have been identified in numerous Arabidopsis CesA superfamily genes, undergoing phenotypic and biochemical characterization.
  • Despite the inability to definitively link specific Csl genes to their polysaccharide products, their expression and localization align with the expected patterns for primary cell wall polysaccharide synthases.

Outlook:

  • Further research will focus on characterizing identified mutations and integrating expression data to assign functions to Csl genes.
  • Comparative genomics across species will help understand the evolutionary conservation and diversification of Csl gene families.
  • Elucidating Csl gene functions is critical for understanding plant cell wall biosynthesis and for potential applications in plant biotechnology and biomass production.