Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Screening for novel cry genes by hybridization.

C E Beard¹, C Ranasinghe, R J Akhurst

¹CSIRO Division of Entomology, GPO Box 1700, Canberra, A.C.T., 2614, Australia.

Letters in Applied Microbiology

|September 14, 2001

Summary

Researchers developed a new method using cry gene sequences to efficiently find novel toxins from Bacillus thuringiensis (Bt). This technique enhances the discovery of diverse toxin genes in a single step.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Translation, cross-cultural adaptation, and validation of the Duke Activity Status Index (DASI) to Sinhala language.

Perioperative medicine (London, England)·2024

Same author

Genetic polymorphism in <i>Methylenetetrahydrofolate Reductase chloride transport protein 6</i> (<i>MTHFR CLCN6</i>) gene is associated with keratinocyte skin cancer in a cohort of renal transplant recipients.

Skin health and disease·2022

Same author

Mucosal IL-4R antagonist HIV vaccination with SOSIP-gp140 booster can induce high-quality cytotoxic CD4<sup>+</sup>/CD8<sup>+</sup> T cells and humoral responses in macaques.

Scientific reports·2020

Same author

A hierarchical role of IL-25 in ILC development and function at the lung mucosae following viral-vector vaccination.

Vaccine: X·2019

Same author

α-PD-1 therapy elevates Treg/Th balance and increases tumor cell pSmad3 that are both targeted by α-TGFβ antibody to promote durable rejection and immunity in squamous cell carcinomas.

Journal for immunotherapy of cancer·2019

Same author

Viral vector and route of administration determine the ILC and DC profiles responsible for downstream vaccine-specific immune outcomes.

Vaccine·2019

Area of Science:

Microbiology
Molecular Biology
Biotechnology

Background:

Bacillus thuringiensis (Bt) produces Cry toxins, which are insecticidal proteins.
Discovering novel Cry toxins is crucial for developing new biopesticides.
Existing methods for identifying Cry toxin genes can be time-consuming and less efficient.

Purpose of the Study:

To develop an efficient and sensitive method for discovering novel Cry toxins.
To improve the screening process for Cry toxin genes in Bacillus thuringiensis.

Main Methods:

Utilized a cocktail of cry gene sequences as a hybridization probe.
Screened Bacillus thuringiensis strains and their gene libraries.
Optimized hybridization and washing conditions to prevent cross-hybridization.

Related Experiment Videos

Main Results:

The method successfully identified diverse cry genes using a mixed probe.
No cross-hybridization was observed between different cry gene families under optimized conditions.
Probes with partial or complete cry gene sequences yielded similar hybridization patterns.

Conclusions:

Hybridization with a mixture of cry gene sequences is an efficient tool for screening gene libraries.
This technique enables the detection of a diverse range of cry genes in a single step.
The developed method significantly enhances the ease and efficiency of discovering novel toxin genes.