Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MNU induction of neoplasia in a platyfish model.

S Kazianis1, I Gimenez-Conti, R B Setlow

  • 1Department of Chemistry and Biochemistry, Southwest Texas State University, San Marcos, Texas 78666, USA.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|September 14, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patients treated for acute VTE during periods of treatment-related thrombocytopenia have high rates of recurrent thrombosis and transfusion-related adverse outcomes.

Journal of thrombosis and thrombolysis·2017
Same author

Is there a need for morphologic exam to detect relapse in AML if multi-parameter flow cytometry is employed?

Leukemia·2017
Same author

Gemtuzumab ozogamicin in acute myeloid leukemia.

Leukemia·2017
Same author

Mitoxantrone, etoposide and cytarabine following epigenetic priming with decitabine in adults with relapsed/refractory acute myeloid leukemia or other high-grade myeloid neoplasms: a phase 1/2 study.

Leukemia·2017
Same author

Measurable residual disease testing in acute myeloid leukaemia.

Leukemia·2017
Same author

Characteristics and outcome of patients with therapy-related acute promyelocytic leukemia front-line treated with or without arsenic trioxide.

Leukemia·2017

This study explores fish genetics and cancer, revealing that a specific gene (CDKN2X) influences melanosis in hybrid Xiphophorus fish after carcinogen exposure. This finding aids in understanding cancer development and potential treatments.

Area of Science:

  • Genetics
  • Cancer Biology
  • Ichthyology

Background:

  • Xiphophorus fish hybrids have long been models for melanoma research.
  • A novel cross between X. maculatus and X. couchianus was established.
  • Specific pigment genes (Sd, Dr, Af) show altered expression in hybrids.

Purpose of the Study:

  • To investigate the genetic basis of melanosis in X. maculatus x X. couchianus hybrids.
  • To determine the effects of N-methyl-N-nitrosourea (MNU) on hybrid fish.
  • To identify genetic factors associated with carcinogen-induced melanosis.

Main Methods:

  • Creation of interspecific hybrid crosses (X. maculatus x X. couchianus).
  • Exposure of backcross 1 (BC1) hybrids to the carcinogen N-methyl-N-nitrosourea (MNU).
Keywords:
Non-programmatic

Related Experiment Videos

  • Phenotypic analysis of pigment patterns and tumor development.
  • Genetic linkage analysis to associate genotypes with phenotypes.

    • Main Results:

    • MNU treatment induced schwannomas, fibrosarcomas, and retinoblastomas.
    • MNU-treated males with the Anal fin spot (Af) gene developed pronounced melanosis.
    • Significant association found between CDKN2X genotype and melanization degree (p < 0.01).

    Conclusions:

    • A specific locus on linkage group V, potentially CDKN2X, plays a role in melanosis development.
    • The study outlines genetic preconditions and a carcinogenic scheme for ventral melanosis in hybrid fish.
    • Xiphophorus fish are valuable models for studying diverse carcinogenic mechanisms beyond melanoma.