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Methylobacillus flagellatus KT contains a novel cbo-type cytochrome oxidase
E V Strom1, T Y Dinarieva, A I Netrusov
1Department of Microbiology, Moscow State University, Lenin's Hills, Moscow 119899, Russia.
FEBS Letters
|September 15, 2001
Abstract:
The o-type oxidase from the methanol-grown obligate methylotroph Methylobacillus flagellatus KT has been purified to homogeneity. The complex is composed of four subunits (57, 40, 35 and 30 kDa). It contains six haems (4C:1B:1O) and one copper atom per molecule. It is proposed that the haem O-Cu(B) binuclear centre and a low-spin haem B are located in subunit I (57 kDa), two haems C reside in the cytochrome c homodimer (35 kDa), two haems C belong to the dihaem cytochrome c (30 kDa). The presented data provide evidence that cytochrome cbo is a novel representative of the haem-copper oxidase superfamily.