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Expression of the Pasteurella multocida ompH gene is negatively regulated by the Fur protein
M Bosch1, R Tarragó, M E Garrido
1Department of Genetics and Microbiology, Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain.
Abstract:
The fur gene of Pasteurella multocida has been cloned by complementation of an Escherichia coli fur mutant. The P. multocida fur gene, which encodes a predicted protein of 147 amino acids, displaying the highest identity (89%) with the same protein of Haemophilus influenzae, is negatively regulated by its own product. By construction of a P. multocida fur mutant, it has been demonstrated that the ompH gene, encoding a major structural protein of the outer membrane, presenting high antigenicity power, is negatively regulated by iron and glucose. Furthermore, wild-type and fur-defective cells of P. multocida show the same level of virulence.
Insights
Researchers cloned the Pasteurella multocida fur gene, finding it regulates itself. A fur mutant showed the ompH gene is controlled by iron and glucose, with no change in virulence.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- The fur gene regulates iron uptake in many bacteria.
- Understanding gene regulation in Pasteurella multocida is crucial for controlling infections.
Purpose of the Study:
- To clone and characterize the Pasteurella multocida fur gene.
- To investigate the regulation of the ompH gene in P. multocida.
- To assess the role of the fur gene in P. multocida virulence.
Main Methods:
- Cloning of the P. multocida fur gene by complementation of an Escherichia coli fur mutant.
- Construction and analysis of a P. multocida fur mutant.
- Analysis of ompH gene regulation by iron and glucose.
Main Results:
- The P. multocida fur gene was cloned, encoding a 147-amino acid protein with high similarity to H. influenzae Fur.
- The P. multocida fur gene is negatively autoregulated.
- The ompH gene is negatively regulated by iron and glucose in P. multocida.
- P. multocida fur mutants exhibited similar virulence to wild-type strains.
Conclusions:
- The P. multocida fur gene plays a role in regulating gene expression, particularly the ompH gene.
- Iron and glucose are key environmental factors influencing ompH expression.
- The fur gene is not essential for P. multocida virulence in the tested conditions.