Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiphoton fluorescence microscopy.

E Gratton1, N P Barry, S Beretta

  • 1Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, 1110 West Green Street, Urbana, Illinois 61801, USA. enrico@scs.uiuc.edu

Methods (San Diego, Calif.)
|September 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions.

Methods and applications in fluorescence·2023
Same author

Experiences of parents and caregivers in pediatric intensive care units: A qualitative study.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2022
Same author

CERKL is upregulated in cutaneous squamous cell carcinoma and maintains cellular sphingolipids and resistance to oxidative stress.

The British journal of dermatology·2021
Same author

CERKL is Upregulated in Cutaneous Squamous Cell Carcinoma and Maintains Cellular Sphingolipids and Resistance to Oxidative Stress.

The British journal of dermatology·2020
Same author

Head down tilt 15° in experimental intracerebral hemorrhage: a randomized noninferiority safety trial.

European journal of neurology·2020
Same author

Thrombolysis and bridging therapy in patients with acute ischaemic stroke and Covid-19.

European journal of neurology·2020
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Multiphoton fluorescence microscopy enables new measurements by exploiting simplified optics. This technique allows detailed analysis of giant vesicle membrane domains and their temperature-dependent structural changes.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Multiphoton fluorescence microscopy offers intrinsic optical sectioning for targeted excitation within cells and tissues.
  • Its simplified emission pathway, lacking an emission pinhole, presents unique advantages over confocal microscopy.
  • This simplification allows for novel measurement strategies not feasible with conventional setups.

Purpose of the Study:

  • To demonstrate novel measurement techniques utilizing the simplified optics of multiphoton microscopy.
  • To investigate phase domain characteristics and dynamics in giant vesicles.
  • To analyze temperature-induced changes in membrane domain geometry and gel formation.

Main Methods:

  • Utilizing dual-emission wavelength measurements for phase domain identification in giant vesicles.

Related Experiment Videos

  • Performing fluctuation experiments at specific membrane locations.
  • Applying dual-wavelength measurements combined with scanning fluctuation analysis.

    • Main Results:

    • Successfully identified distinct phase domains within giant vesicles.
    • Quantified membrane domain geometry changes and incipient gel domain formation upon cooling.
    • Demonstrated the feasibility of localized fluctuation experiments on vesicle membranes.

    Conclusions:

    • The simplified optical path of multiphoton microscopy enables advanced analytical capabilities.
    • Dual-wavelength measurements are effective for characterizing membrane domain behavior and phase transitions.
    • This approach provides new insights into the physical properties of biological membranes.