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Novel cis-acting replication element in the adeno-associated virus type 2 genome is involved in amplification of
1Laboratoire de Thérapie Génique, CHU Hotel Dieu, 44035 Nantes Cedex 01, France.
Journal of Virology
|September 18, 2001
Abstract:
This study identifies a region of the adeno-associated virus type 2 (AAV-2) rep gene (nucleotides 190 to 540 of wild-type AAV-2) as a cis-acting Rep-dependent element able to promote the replication of transiently transfected plasmids. This viral element is also shown to be involved in the amplification of integrated sequences in the presence of adenovirus and Rep proteins.