Electrophoretic investigation of some dehydrogenases in Leishmania amastigotes

Amastigotes of Leishmania mexicana mexicana and of Leishmania donovani were grown in mouse peritoneal macrophages and dog sarcoma cells in culture. Polyacrylamide gel disc electrophoresis of amastigote-infected tissue cultures failed to detect parasite lactate dehydrogenase and malate dehydrogenase, although the presence of glucose-6-phosphate dehydrogenase was indicated.