Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Utilization of tmRNA sequences for bacterial identification.

W Schönhuber1, G Le Bourhis, J Tremblay

  • 1Lehrstuhl für Mikrobielle Okologie, Universität Konstanz, Fach M654, Universitätsstrasse 10, D-78457 Konstanz, Germany. wilhelm.schoenhuber@uni-konstanz.de

BMC Microbiology
|September 19, 2001
PubMed
Summary

Transfer messenger RNA (tmRNA) offers a novel approach for bacterial identification, overcoming limitations of ribosomal RNA. This stable RNA molecule enables specific in situ visualization and phylogenetic assignment of bacteria, even within species.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using phenotyping to visualize and identify selfish bacteria: a methods guide.

Microbiology spectrum·2025
Same author

Assessment of the safety of Levilactobacillus brevis CNCM I-5321, a probiotic candidate strain isolated from pulque with anti-proliferative activities.

Beneficial microbes·2024
Same author

Diastolic Blood Pressure and Cognitive Functioning: Differences by Systolic Blood Pressure Among US Adults.

American journal of Alzheimer's disease and other dementias·2023
Same author

Over-production of exopolysaccharide by Lacticaseibacillus rhamnosus CNCM I-3690 strain cutbacks its beneficial effect on the host.

Scientific reports·2023
Same author

Integrative couple treatment for pathological gamblers with an emphasis on forgiveness processes: A case study with three couples.

Journal of marital and family therapy·2022
Same author

2021 Canadian Surgery Forum: Virtual, online Sept. 21-24, 2021.

Canadian journal of surgery. Journal canadien de chirurgie·2022

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Phylogenetics

Background:

  • Ribosomal RNA (rRNA) is commonly used for bacterial identification but faces limitations in species-level discrimination and probe accessibility.
  • Fluorescence in situ hybridization (FISH) using rRNA probes can be hindered by sequence conservation and accessibility issues.
  • Transfer messenger RNA (tmRNA), encoded by the ssrA gene, is a stable RNA involved in protein degradation and presents an alternative for bacterial identification.

Purpose of the Study:

  • To evaluate tmRNA as a target for in situ identification and phylogenetic analysis of bacteria.
  • To overcome the limitations of 16S rRNA in bacterial species discrimination and FISH probe accessibility.
  • To develop methods for amplifying and visualizing tmRNA in diverse bacterial species.

Main Methods:

Related Experiment Videos

  • Designed universal primers targeting conserved regions of tmRNA genes for amplification from Gram-positive bacteria.
  • Performed sequence analysis of amplified tmRNAs for phylogenetic assignment.
  • Developed and tested FISH probes targeting tmRNA for specific bacterial visualization, using E. coli as a model.

Main Results:

  • Successfully amplified and sequenced tmRNA from various Gram-positive bacteria, enabling phylogenetic analysis.
  • Demonstrated significant inter-species sequence divergence in tmRNA, particularly in genera like Listeria, aiding species-level identification.
  • Showcased specific in situ visualization of bacteria using tmRNA-targeted FISH probes.

Conclusions:

  • tmRNA is a promising molecule for bacterial identification due to its presence across diverse bacterial species.
  • Conserved regions in tmRNA genes facilitate primer design for broad applicability.
  • The potential of tmRNA as a FISH target offers a valuable tool for bacterial in situ identification and phylogenetic studies.