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Synthesis and study of the fluorescein conjugate of the nucleotide dPTP
W J Cummins1, A L Hamilton, C L Smith
1Amersham Pharmacia Biotech UK Limited, Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA, England.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
The synthesis of a fluorescein conjugate on the non-natural base P is described. The ability of the newly synthesised fluorescein--dPTP and of a fluorescein-11-dUTP to compete with the natural nucleotide TTP was also studied. Overall the efficiency of labelling a nucleic acid with a fluorescein moiety was found to be approximately equal.