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Novel nucleoside triphosphate analogs for the enzymatic labeling of nucleic acids
A D Barone1, C Chen, G H McGall
1Affymetrix, Inc., Santa Clara, California, USA.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
We have evaluated several novel nucleotide analogs suitable for enzymatic labeling of nucleic acid targets for a variety of array-based assays. Two new reagents in particular, a C4-labeled 1-(2',3'-dideoxy-beta-D-ribofuranosyl) imidazole-4-carboxamide 5'-triphosphate 5 and an N1-labeled 5-(beta-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione 5'-triphosphate 3, were found to be excellent substrates for labeling with terminal deoxynucleotidyl transferase and T7 RNA polymerase, respectively.