Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A DNA-binding peptide from a phage display library.

J Wölcke1, E Weinhold

  • 1Max-Planck-Instiut für molekulare Physiologie, Abteilung Physikalische Biochemie, Otto-Hahn-Str. 11, D-44227 Dortmund, Germany.

Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Miniaturized HTS technologies - uHTS.

Drug discovery today·2001
Same author

The mechanism of DNA cytosine-5 methylation. Kinetic and mutational dissection of Hhai methyltransferase.

The Journal of biological chemistry·2001
Same author

Structure of the N6-adenine DNA methyltransferase M.TaqI in complex with DNA and a cofactor analog.

Nature structural biology·2001
Same author

Functional roles of the conserved threonine 250 in the target recognition domain of HhaI DNA methyltransferase.

The Journal of biological chemistry·2000
Same author

Reviving a dead enzyme: cytosine deaminations promoted by an inactive DNA methyltransferase and an S-adenosylmethionine analogue.

Biochemistry·2000
Same author

Identification of the binding site for the extrahelical target base in N6-adenine DNA methyltransferases by photo-cross-linking with duplex oligodeoxyribonucleotides containing 5-iodouracil at the target position.

The Journal of biological chemistry·1999
Same journal

LncRNA EGOT inhibits the proliferation of lung adenocarcinoma cells and is associated with poor prognosis.

Nucleosides, nucleotides & nucleic acids·2026
Same journal

Can the combination of 5-fluorouracil and gemcitabine increase the treatment efficacy of pancreatic and colon cancer?

Nucleosides, nucleotides & nucleic acids·2026
Same journal

"Aptamer-guided therapeutics and diagnostics for metabolic syndrome: Design, delivery, and translation of aptamer-based systems".

Nucleosides, nucleotides & nucleic acids·2026
Same journal

The impact of vitamin B12 deficiency on urinary profile of <i>de novo</i> purine synthesis intermediates: consequences of the methylfolate trap.

Nucleosides, nucleotides & nucleic acids·2026
Same journal

Evaluation of MTH1 and DCTPP1 inhibitors in fluorocyclopentenylcytosine (RX-3117) resistant lung cancer cells and potential cross resistance patterns.

Nucleosides, nucleotides & nucleic acids·2026
Same journal

Circular RNAs: promising biomarkers for diagnosis, prognosis and therapy in oral squamous cell carcinoma.

Nucleosides, nucleotides & nucleic acids·2026
See all related articles

Researchers identified a specific DNA-binding peptide using phage display technology. This peptide, with the sequence SVSVGMKPSPRP, demonstrated binding to DNA, confirmed through enzyme-linked immunosorbent assay (ELISA).

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Protein Engineering

Background:

  • Phage display is a powerful technique for discovering novel protein functionalities.
  • Identifying specific DNA-binding peptides is crucial for various biotechnological applications.
  • Enzyme-linked immunosorbent assay (ELISA) is a standard method for detecting protein-DNA interactions.

Purpose of the Study:

  • To select and characterize a novel DNA-binding peptide from a random peptide library.
  • To utilize DNA methyltransferase M.TaqI and a specific oligonucleotide for peptide selection.
  • To confirm the DNA-binding ability of the selected peptide.

Main Methods:

  • Random peptide phage display library screening.
  • Competitive elution using biotin-labeled M.TaqI recognition sequence (5'-TCGA-3').

Related Experiment Videos

  • Enzyme-linked immunosorbent assay (ELISA) for DNA-binding confirmation.

    • Main Results:

    • A specific DNA-binding peptide sequence (SVSVGMKPSPRP) was identified in nine out of ten selected phages.
    • The selected phage clone demonstrated specific binding to DNA.
    • The selection process successfully employed M.TaqI and a complementary oligonucleotide.

    Conclusions:

    • A novel DNA-binding peptide was successfully isolated using a directed selection strategy.
    • The identified peptide holds potential for applications in molecular biology and biotechnology.
    • Phage display remains an effective method for discovering functional peptides with specific DNA-binding properties.