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Design and properties of hepatitis C virus antisense oligonucleotides for liver specific drug targeting
T J Lehmann1, M Serwe, W H Caselmann
1Institute of Organic Chemistry, Johann Wolfgang Goethe-University, Marie-Curie-Strasse 11, D-60439 Frankfurt am Main, Germany.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
Different backbone modified antisense oligonucleotides (AS-ODNs) directed against the hepatitis C virus genome were 5'-conjugated to cholesterol, cholic acid or taurocholic acid to enhance liver specific drug targeting and hepatocellular uptake. The lipophilic character of modified AS-ODNs was determined from RP-HPLC retention times and duplex stability was correlated with Tm-values from UV melting curves.