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Fluorescence resonance energy transfer dye nucleotide terminators: a new synthetic approach for high-throughout DNA
S Nampalli1, M Khot, J R Nelson
1Amersham Pharmacia Biotech, Piscataway, New Jersey 08855, USA.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.