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DNA triple-helix formation at pyrimidine-purine inversion sites
S P Parel1, J Marfurt, C J Leumann
1Department of Chemistry and Biochemistry, University of Bern, CH-3012 Bern, Switzerland.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
A systematic investigation of a series of triplex forming oligonucleotides (TFOs) containing alpha-, and beta-thymidine, alpha- and beta-N7-hypoxanthine, and alpha- and beta-N7 and N9 aminopurine nucleosides, designed to bind to T-A inversion sites in DNA target sequences was performed. Data obtained from gel mobility assays indicate that T-A recognition in the antiparallel triple-helical binding motif is possible if the nucleoside alpha N9-aminopurine is used opposite to the inversion site in the TFO.