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2'-modified oligonucleotides from methoxyoxalamido and succinimido precursors: synthesis, properties, and
N Polushin1, A Malykh, O Malykh
1Fidelity Systems, Inc., 7961 Cessna Avenue, Gaithersburg, Maryland 20879-4117, USA.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
Synthesis of 2'-modified oligonucleotides from 2'-methoxyoxalamido (MOX) and 2'-succinimido (SUC) precursors is described. Their physical and biochemical properties were assessed. Synthesized oligonucleotides were used as primers in advanced DNA sequencing protocols. An example of sequencing directly off genomic DNA template without prior cloning or PCR amplification is presented.