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Interaction of LNA oligonucleotides with mdr1 promoter
E L Chernolovskaya1, A A Koshkin, V V Vlassov
1Novosibirsk Institute of Bioorganic Chemistry, Lavrentiev ave., 8, Novosibirsk, 630090, Russia. elena_ch@niboch.nsc.ru
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
LNA oligonucleotides [1] can be used for targeting to double stranded DNA by the "strand invasion" mechanism. We used affinity modification by reactive oligonucleotide conjugates for investigation of oligonucleotides interaction with structured DNA. The tested LNAs and oligonucleotides of the same sequence were assayed as anti-mdr1 drugs in different cell cultures. One of the oligos, LNA79 strongly inhibited mdr1 induction in Hela cells and totally prevented activation of mdr1 in K-562.