Related Experiment Video
Updated: Aug 10, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Published on: March 14, 2021
Lysophosphatidic acid activates the 70-kDa S6 kinase via the lipoxygenase pathway
F S Willard1, L A Berven, M F Crouch
1Molecular Signalling Group, Australian National University, Canberra, ACT 2601, Australia. francis.willard@anu.edu.au
Abstract:
Many hormones are known to activate the 70-kDa S6 kinase (p70(S6K)). The signalling pathways mediating p70(S6K) activation are only partially characterized. We investigate, in this report, the mechanisms by which lysophosphatidic acid (LPA) activates p70(S6K). We observed that p70(S6K) activation was conventional, in that it was sensitive to both rapamycin and PI3 kinase inhibition. p70(S6K) activation appeared to be caused by the activation of several phospholipase pathways. LPA was an effective stimulus of phospholipase C induced intracellular calcium mobilization, which appeared to participate in p70(S6K) activation. Similarly, the effect of LPA on p70(S6K) activity was antagonized by butan-1-ol but not butan-2-ol suggesting the involvement of agonist stimulated phospholipase D activity. Further, antagonism of the phospholipase A(2) and lipoxygenase pathways attenuated p70(S6K) activation indicating a novel mechanism of p70(S6K) regulation. We conclude that in Swiss 3T3 cells LPA coordinates activation of several phospholipases to regulate p70(S6K).
More Related Videos
12:27A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
Published on: June 8, 2022
10:31A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
Published on: September 26, 2025
Related Concept Videos
Amplifying Signals via Second Messengers
Amplifying Signals via Enzymatic Cascade
MAPK Signaling Cascades
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway
IP3/DAG Signaling Pathway