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Related Experiment Videos

Putative DNA-(amino)methyltransferases in eucaryotes.

B Y Shorning1, B F Vanyushin

  • 1Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899, Russia.

Biochemistry. Biokhimiia
|September 21, 2001
PubMed
Summary

Computer analysis revealed homologous DNA-(amino)methyltransferase genes in diverse eukaryotes, including humans and plants. These genes are located in nuclear DNA, suggesting conserved functions across species.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • DNA-(amino)methyltransferases are crucial enzymes in prokaryotes.
  • The presence and function of these enzymes in eukaryotes are less understood.

Purpose of the Study:

  • To identify eukaryotic homologs of prokaryotic DNA-(amino)methyltransferases.
  • To determine the genomic location of these putative eukaryotic genes.

Main Methods:

  • Bioinformatic analysis of known databases.
  • Comparative analysis of open reading frames (ORFs) and conserved motifs.
  • Genomic localization studies (nuclear vs. mitochondrial DNA).

Main Results:

  • Homologous open reading frames (ORFs) for DNA-(amino)methyltransferases were found in Leishmania major, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Arabidopsis thaliana, Drosophila melanogaster, Caenorhabditis elegans, and Homo sapiens.

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  • Conserved motifs characteristic of bacterial DNA-(amino)methyltransferases were identified in the putative eukaryotic protein sequences.
  • All identified eukaryotic DNA-(amino)methyltransferase ORFs are encoded in nuclear DNA, with no homologous sequences found in mitochondrial genomes.

    • Conclusions:

    • Eukaryotic genomes contain nuclear DNA sequences homologous to prokaryotic DNA-(amino)methyltransferases.
    • The presence of these conserved genes suggests potentially important, yet uncharacterized, functions in eukaryotes.
    • Further investigation into these proteins, particularly adenine DNA-methyltransferases, is warranted.