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Related Experiment Videos

Long-term intrathecal catheterization in the rat.

L Jasmin1, P T Ohara

  • 1Department of Neurological Surgery, UCSF, Box 0112, 505 Parnassus Ave., San Francisco, CA 94143, USA. ucpain@itsa.ucsf.edu

Journal of Neuroscience Methods
|September 21, 2001
PubMed
Summary

We developed a novel intrathecal catheter system for long-term drug delivery in awake rats. This system enables repeated administration of substances, proving effective for chronic behavioral and pharmacological studies.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Surgical techniques

Background:

  • Chronic intrathecal drug administration is crucial for long-term studies.
  • Existing methods may be invasive or unsuitable for prolonged use in awake animals.

Purpose of the Study:

  • To develop and validate a reliable intrathecal catheter system for repeated drug delivery in awake rats over extended periods.
  • To assess the system's biocompatibility, functional longevity, and utility in neuropharmacological research.

Main Methods:

  • A polyurethane catheter was surgically implanted in the sacral subarachnoid space of rats.
  • The catheter was tunneled subcutaneously and exteriorized via a titanium port for repeated administration.
  • System function was verified using lidocaine injections and neurochemical assays (DBH-SAP).

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Main Results:

  • The catheter system was well-tolerated, causing no sensory or motor deficits and allowing normal housing and behavior.
  • Catheter functionality was confirmed over 9 months, with 60-83% remaining functional at various time points.
  • Intrathecal administration of DBH-SAP successfully induced noradrenergic denervation.

Conclusions:

  • This intrathecal catheter system provides a robust platform for long-term, repeated drug administration in awake rats.
  • The device is suitable for chronic behavioral and pharmacological investigations, including neurochemical manipulations.