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Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Published on: February 22, 2020
O-specific polysaccharide of the marine bacterium "Alteromonas marinoglutinosa" NCIMB 1770
N A Komandrova1, S V Tomshich, V V Isakov
1Pacific Institute of Bioorganic Chemistry, Far East Division of the Russian Academy of Sciences, Vladivostok, 690022 Russia. piboc@stl.ru
Abstract:
The O-specific polysaccharide of the marine bacterium "Alteromonas marinoglutinosa" NCIMB 1770 was obtained by mild acid degradation of the corresponding lipopolysaccharide and found to contain D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-mannosamine residues in equimolar ratio. Based on methylation analysis, periodate oxidation, and 13C-NMR spectroscopy data of native and modified polysaccharides, the following structure of the trisaccharide repeating unit of the O-specific polysaccharide was established: [structure: see text]
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