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An improved gas distribution system for anaerobic screening of multiple microbial cultures
1Lundberg Laboratory, Department of Molecular Biotechnology, Chalmers University of Technology, P.O. Box 462, S-405 30 Göteborg, Sweden. hadi.valadi@molbiotech.chalmers.se
Abstract:
A cultivation set-up for multiple cultures has been designed that can be used for anaerobic screening for quantitative changes in growth rate or other analyses, e.g. protein composition of different strains. The developed gas distribution system provides a reproducible level of anaerobicity in 30 cultivation flasks and resembles the open system of a high-performance bioreactor in that it ensures cultivation at atmospheric pressure and avoids supersaturation of carbon dioxide. The system is cheap and user-friendly and allows rapid screenings of many strains simultaneously.