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Kinetics of photo-active bacteriorhodopsin analog 3,4-didehydroretinal
T Jussila1, N V Tkachenko, S Parkkinen
1Institute of Materials Chemistry, Tampere University of Technology, P.O. Box 541, Tampere, Finland. tapio.jussila@tut.fi
Journal of Photochemistry and Photobiology. B, Biology
|September 22, 2001
Abstract:
Kinetics of the photo-induced processes of the transient states of the 3,4-didehydroretinal (3,4-dhr) modified bacteriorhodopsin (bR) was studied by a flash photolysis method in a water suspension at room temperature. The excitation initiated a photocycle with several transient intermediates similar to the trans photocycle of native bR. The main observation of the study was that although major part (80%) of the population of the M state relaxed via the O intermediate as in natural bR, 20% relaxed directly to the bR ground state in 200 ms.