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Related Experiment Video

Updated: Jul 11, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

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RpoS-dependent transcriptional control of sprE: regulatory feedback loop.

N Ruiz¹, C N Peterson, T J Silhavy

¹Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

Journal of Bacteriology

|September 22, 2001

Summary

Escherichia coli

Area of Science:

Microbiology
Molecular Biology
Bacterial Physiology

Background:

Nutrient starvation triggers stationary-phase responses in E. coli.
The ClpXP protease regulates RpoS stability, a key sigma factor.
SprE regulates RpoS proteolysis, but its own regulation is unclear.

Purpose of the Study:

To elucidate the in vivo regulation of the SprE response regulator.
To investigate the transcriptional control of SprE expression.
To determine the role of RssA in SprE and RpoS regulation.

Main Methods:

Investigated RpoS-dependent gene expression using transcriptional analysis.
Analyzed operon structure and promoter activity of the rssA-sprE locus.
Utilized in-frame deletion mutants to assess gene function.

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Main Results:

RpoS positively regulates sprE transcription, increasing SprE levels.
Identified an additional RpoS-dependent promoter in the rssA-sprE intergenic region.
RssA does not influence SprE or RpoS levels or regulation.

Conclusions:

SprE accumulation during stationary phase is transcriptionally controlled by RpoS.
The rssA-sprE operon and an intergenic promoter contribute to SprE regulation.
RssA is not essential for SprE or RpoS homeostasis in E. coli.