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Updated: Aug 14, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Published on: May 16, 2017
Using electron-microscopy images as a model for molecular replacement
1York Structural Biology Laboratory, Chemistry Department, University of York, York YO10 5DD, England. e.dodson@ysbl.york.ac.uk
Abstract:
This review addresses the technical problems encountered while using models based on electron microscopy to generate initial phases for crystallographic studies. The test cases used were the gp6 portal protein with 13-fold rotational symmetry and the human hepatitis virus HepB, a viral assembly with T = 4 icosohedral symmetry.
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