Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analyzing and correcting spectrometer temperature sensitivity.

P J Bowyer1, A G Swanson, G A Morris

  • 1Department of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|September 25, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rheo-dissolution: A new platform for the simultaneous measurement of rheology and drug release.

Carbohydrate polymers·2019
Same author

Structure and physicochemical properties of Ghanaian grewia gum.

International journal of biological macromolecules·2018
Same author

Structure-Function Relationships in Pectin Emulsification.

Food biophysics·2018
Same author

Relaxation-encoded NMR experiments for mixture analysis: REST and beer.

Chemical communications (Cambridge, England)·2017
Same author

Structural characterisation and rheological properties of a polysaccharide from sesame leaves (Sesamum radiatum Schumach. & Thonn.).

Carbohydrate polymers·2016
Same author

Increasing the quantitative bandwidth of NMR measurements.

Chemical communications (Cambridge, England)·2016
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

Room temperature fluctuations significantly impact Nuclear Magnetic Resonance (NMR) experiments, causing spectral artifacts. Suppressing these temperature sensitivities improved spectrometer performance over tenfold.

Area of Science:

  • Spectroscopy
  • Nuclear Magnetic Resonance (NMR)

Background:

  • Spectrometer stability and reproducibility are critical for modern NMR experiments.
  • Room temperature variations are a key source of instability, inducing coherent artifacts in NMR spectra.
  • Temperature changes affect NMR signal phases, amplitudes, and frequencies, leading to artifacts like t(1)-noise and incomplete signal cancellation.

Purpose of the Study:

  • To identify, quantify, and suppress sources of temperature sensitivity in a 300-MHz NMR spectrometer.
  • To improve the signal-to-artifact ratio in NMR spectra affected by temperature fluctuations.

Main Methods:

  • Investigated the impact of room temperature variations on NMR signal characteristics.
  • Quantified temperature-induced artifacts, including F(1) satellite signals.

Related Experiment Videos

  • Developed and implemented methods to suppress temperature sensitivity.

    • Main Results:

    • Demonstrated that small temperature changes cause significant alterations in NMR signal parameters.
    • Identified air conditioning as a cause of oscillating room temperatures, leading to specific artifacts like "parallel diagonals" in 2D NMR.
    • Achieved a greater than 10-fold improvement in the signal-to-artifact ratio.

    Conclusions:

    • Controlling room temperature is essential for high-quality NMR data acquisition.
    • Effective suppression of temperature sensitivity significantly enhances spectrometer performance and data reliability.
    • The findings are crucial for optimizing NMR experiments in environments with fluctuating temperatures.