Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spinal Cord Neuron Isolation: A Density Gradient Procedure to Isolate Neonatal Spinal Cord Neurons for In Vitro Studies06:10

Spinal Cord Neuron Isolation: A Density Gradient Procedure to Isolate Neonatal Spinal Cord Neurons for In Vitro Studies

3.2K
Source: Eldeiry, M. et al. Spinal Cord Neurons Isolation and Culture from Neonatal Mice. J. Vis. Exp. (2017)In this video, we describe a step-by-step protocol for isolating spinal cord neurons from a neonatal mouse for in vitro...
3.2K
Targeted Viral Delivery to Corticospinal Neurons in a Neonatal Rat to Study Spinal Cord Injury02:32

Targeted Viral Delivery to Corticospinal Neurons in a Neonatal Rat to Study Spinal Cord Injury

236
Source: Smit, R. D., et al. Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery. J. Vis. Exp. (2021)This video demonstrates the targeted delivery of viral vectors to corticospinal neurons in a neonatal rat. The protocol enables precise gene expression using anterograde and retrograde viral injections, facilitating research on neural regeneration and plasticity following spinal cord...
236
Activity-based Training on a Treadmill with Spinal Cord Injured Wistar Rats06:40

Activity-based Training on a Treadmill with Spinal Cord Injured Wistar Rats

8.5K
This protocol demonstrates our model of activity-based locomotor treadmill training for rats with spinal cord injury (SCI). Included is both quadrupedal and forelimb-only groups, in addition to two distinct types of non-trained control groups. Investigators are able to assess training effects on SCI rats using this...
8.5K
Spinal Cord Neurons Isolation and Culture from Neonatal Mice07:49

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

20.2K
This study presents a technique for the isolation of neurons from WT neonatal mice. It requires the careful dissection of the spinal cord from the neonatal mouse, followed by the separation of neurons from the spinal cord tissue through mechanical and enzymatic...
20.2K
A Neonatal Mouse Spinal Cord Compression Injury Model13:31

A Neonatal Mouse Spinal Cord Compression Injury Model

13.1K
This article describes a method for generating a reproducible spinal cord compression injury (SCI) in the neonatal mouse. The model provides an advantageous platform for studying mechanisms of adaptive plasticity that underlie spontaneous functional recovery.
13.1K
Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats08:57

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

24.3K
We describe two tactile sensory testing methods for acute or chronic periods of spinal cord injury in rats. These validated procedures can detect the development and maintenance of allodynia-like...
24.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk of non-Hodgkin's lymphoma and residential exposure to air pollution in an industrial area in northern Italy: a case-control study.

Archives of environmental & occupational health·2013
Same author

Neural cell-cell and cell-substrate adhesion through N-cadherin, N-CAM and L1.

Journal of neural engineering·2011
Same author

The axonal response after ventral root avulsions at the cervical 7 level in the cat spinal cord.

Restorative neurology and neuroscience·2011
Same author

Ventral root avulsion versus transection at the cervical 7 level of the cat spinal cord.

Restorative neurology and neuroscience·2011
Same author

The pedunculopontine nucleus as an additional target for deep brain stimulation.

Neural networks : the official journal of the International Neural Network Society·2011
Same author

Inhibition of neuronal cell-cell adhesion measured by the microscopic aggregation assay and impedance sensing.

Journal of neural engineering·2010

Related Experiment Video

Updated: Jan 20, 2026

Targeted Viral Delivery to Corticospinal Neurons in a Neonatal Rat to Study Spinal Cord Injury
02:32

Targeted Viral Delivery to Corticospinal Neurons in a Neonatal Rat to Study Spinal Cord Injury

Published on: August 28, 2025

236

Lumbar spinal cord explants from neonatal rat display age-related decrease of outgrowth in culture.

J M Mouveroux1, E A Lakke, E Marani

  • 1Neuroregulation Group, Department of Neurosurgery, Leiden University Medical Centre, The, Leiden, Netherlands.

Neuroscience Letters
|September 25, 2001
PubMed
Summary

Neurite outgrowth from neonatal rat spinal cord explants decreases significantly with age. This study quantifies the relationship between explant age and neurite growth in a collagen matrix.

More Related Videos

Spinal Cord Neurons Isolation and Culture from Neonatal Mice
07:49

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

Published on: July 11, 2017

20.2K
Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats
08:57

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Published on: April 4, 2012

24.3K

Related Experiment Videos

Last Updated: Jan 20, 2026

Targeted Viral Delivery to Corticospinal Neurons in a Neonatal Rat to Study Spinal Cord Injury
02:32

Targeted Viral Delivery to Corticospinal Neurons in a Neonatal Rat to Study Spinal Cord Injury

Published on: August 28, 2025

236
Spinal Cord Neurons Isolation and Culture from Neonatal Mice
07:49

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

Published on: July 11, 2017

20.2K
Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats
08:57

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Published on: April 4, 2012

24.3K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neonatal rat spinal cord explants are used to study neurite outgrowth.
  • Understanding factors influencing neurite growth is crucial for regenerative medicine.

Purpose of the Study:

  • To investigate the relationship between the age of neonatal rat lumbar spinal cord explants and their neurite outgrowth capacity.
  • To determine the factors contributing to age-related changes in neurite outgrowth in vitro.

Main Methods:

  • Lumbar spinal cord explants from rat pups (postnatal day 0-7) were cultured in a chemically defined medium within a collagen matrix for 48 hours.
  • Neurite outgrowth into the surrounding matrix was quantified.
  • Age-matched cortical explants served as controls.

Main Results:

  • A significant inverse correlation was observed between explant age and the number of outgrowing neurites.
  • Neurite outgrowth decreased sharply with increasing age of the explant.
  • The relationship was quantified by the expression log(N)= -0.652 A+17, where N is the number of neurites and A is the age in gestational days.

Conclusions:

  • The age of the neonatal rat spinal cord explant is a critical determinant of neurite outgrowth in vitro.
  • The observed decline in outgrowth is not attributed to myelination or lack of trophic support but potentially to reduced neurite-collagen interaction.
  • These findings suggest developmental changes in spinal neurites affect their ability to interact with the extracellular matrix.