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Microvolume blood-sampling device with low hemolysis and high consistent yield of serum components
Y Tanaka1, Y Noda, M Kobayashi
1Market Development Group, PLUSCARE Division, Arkray Inc., 57 Nishi-aketa-cho, Higashi-kujo, Kyoto, Japan 601-8045. tanakayos@arkray.co.jp
Background:
Blood sampling by finger puncture is convenient, but the need for centrifugation and the problem of hemolysis remain, as does instability when samples must be shipped for analysis. We aimed to develop a blood-sampling device that provided high yields of serum with limited hemolysis and enabled preservation of serum components for at least 7 days at room temperature.
Methods:
For separation of blood cells, we devised a grooved, asymmetric, polysulfonate membrane impregnated with sucrose. We evaluated hemoglobin (Hb) concentrations in the serum, assay values for 15 frequently measured serum components (including glucose), and the stability of analytes in the device.
Results:
In sera from the new device, the Hb concentration was
Conclusions:
The newly developed device allows recovery of 60% of serum components from microvolumes of blood by finger puncture with neither degradation of analytes at room temperature nor hemolysis.