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Real-time PCR assay with fluorescent hybridization probes for rapid interleukin-6 promoter (-174G-->C) genotyping

T Bertsch, W Zimmer, W Casarin

    Clinical Chemistry
    |September 25, 2001
    PubMed
    Abstract

    No abstract available in PubMed .

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    Real Time RT-PCR

    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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