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Related Experiment Videos

Assessment of cisplatin-induced nephrotoxicity by microarray technology.

Q Huang1, R T Dunn, S Jayadev

  • 1Department of Toxicology, Boehringer Ingelheim Pharmaceuticals, Inc., 900 Ridgebury Road, P.O. Box 368, Ridgefield, Connecticut 06877-0368, USA. qhuang@rdg.boeringer-ingelheim.com

Toxicological Sciences : an Official Journal of the Society of Toxicology
|September 25, 2001
PubMed
Summary

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Microarrays revealed cisplatin causes kidney damage and apoptosis in rats. This study highlights microarray utility in toxicology and cautions against direct extrapolation of in vitro to in vivo findings.

Area of Science:

  • Toxicology
  • Molecular Biology
  • Genomics

Background:

  • Cisplatin is a widely used chemotherapy agent with known nephrotoxicity.
  • Understanding the molecular mechanisms of cisplatin-induced kidney damage is crucial for developing protective strategies.
  • Microarray technology offers a powerful tool for global gene expression analysis in toxicological studies.

Purpose of the Study:

  • To investigate gene expression patterns associated with cisplatin-mediated nephrotoxicity using microarrays.
  • To elucidate the biochemical pathways involved in cisplatin toxicity in vivo and in vitro.
  • To assess the utility of microarrays in toxicological research.

Main Methods:

  • Sprague-Dawley rats were administered cisplatin or transplatin, and gene expression was analyzed using rat-specific toxicity arrays.

Related Experiment Videos

  • Histopathological evaluation was performed to assess tissue damage.
  • TaqMan analyses were used to confirm select gene expression changes.
  • In vitro studies were conducted using NRK-52E kidney epithelial cells and clone-9 liver cells.

    • Main Results:

    • Cisplatin administration for 7 days induced renal proximal tubular necrosis and significant gene expression changes in the kidney, but not the liver.
    • Gene expression patterns suggested cisplatin triggers apoptosis and disrupts calcium homeostasis, while also inducing multidrug resistance and tissue regeneration markers.
    • In vitro studies indicated liver cells were more sensitive to cisplatin than kidney cells, contrasting with in vivo findings.

    Conclusions:

    • Microarray analysis effectively identified molecular pathways involved in cisplatin nephrotoxicity, including apoptosis and cellular repair mechanisms.
    • The study demonstrates the value of microarrays in toxicological research for deciphering complex biological responses.
    • Discrepancies between in vivo and in vitro results emphasize the need for careful interpretation when extrapolating data between systems.