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RNA binding proteins and selenocysteine.

P R Copeland1, D M Driscoll

  • 1Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA. copelap@ccf.org

Biofactors (Oxford, England)
|September 25, 2001
PubMed
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Selenocysteine insertion into proteins requires specific RNA-binding proteins (SBPs). This review details the history of SBPs and focuses on SBP2, the essential protein for selenocysteine incorporation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Selenocysteine is a unique amino acid incorporated into proteins via a specialized co-translational mechanism.
  • This process involves cis-acting elements and trans-acting factors, including RNA-binding proteins (SBPs).
  • SBPs interact with the selenocysteine insertion sequence (SECIS) in the 3' untranslated regions of selenoprotein mRNAs.

Purpose of the Study:

  • To review the history and evolution of RNA-binding proteins (SBPs) involved in selenocysteine incorporation.
  • To provide a detailed description of SBP2, a key trans-acting factor.
  • To discuss the potential mechanism of SBP2 in facilitating selenocysteine insertion.

Main Methods:

  • Literature review of SBPs and selenocysteine incorporation mechanisms.

Related Experiment Videos

  • Detailed analysis of SBP2 structure and function.
  • Discussion of experimental evidence and proposed models for SBP2 action.

    • Main Results:

    • SBPs are crucial trans-acting factors for selenocysteine insertion.
    • SBP2 is identified as the sole essential SBP for this process.
    • The review explores the specific roles and interactions of SBP2.

    Conclusions:

    • SBP2 plays a critical and indispensable role in selenocysteine incorporation.
    • Understanding SBP2's mechanism provides insights into the regulation of selenoprotein synthesis.
    • Further research into SBP2 function is warranted to fully elucidate selenocysteine insertion pathways.