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Related Experiment Videos

Microfabricated polycarbonate CE devices for DNA analysis.

Y Liu1, D Ganser, A Schneider

  • 1Physical Sciences Research Laboratories, Motorola Labs, Motorola, Inc., Tempe, Arizona 85284, USA.

Analytical Chemistry
|September 25, 2001
PubMed
Summary
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This study presents plastic microchip capillary electrophoresis (CE) devices fabricated from polycarbonate. UV treatment enhanced fluid transport and electroosmotic flow (EOF), enabling successful DNA separation and on-chip PCR/CE analysis.

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Microchip capillary electrophoresis (CE) offers miniaturized analytical capabilities.
  • Polycarbonate (PC) is a promising material for fabricating microfluidic devices due to its processability.
  • Challenges exist in achieving efficient fluid transport and electroosmotic flow (EOF) in native PC microchannels.

Purpose of the Study:

  • To develop and characterize polycarbonate-based microchip CE devices.
  • To investigate methods for enhancing fluid transport and EOF in PC microchannels.
  • To demonstrate the feasibility of DNA separation and integrated PCR/CE analysis on these plastic devices.

Main Methods:

  • Polycarbonate microchip CE devices were fabricated using compression molding and thermal bonding.

Related Experiment Videos

  • Hydrophobic PC surfaces were treated with UV irradiation to enhance hydrophilicity.
  • Fluid transport and EOF were characterized under varying conditions.
  • DNA separation and on-chip polymerase chain reaction (PCR)/CE were performed.

    • Main Results:

    • Robust thermal bonds were achieved, withstanding forces up to 150 psi.
    • UV irradiation significantly increased surface hydrophilicity and electroosmotic flow (EOF) in PC channels.
    • High-resolution, reproducible DNA separations were demonstrated.
    • Successful on-chip PCR/CE analysis of bacteriophage lambda DNA was achieved.

    Conclusions:

    • UV-treated polycarbonate microchip CE devices provide a viable platform for microfluidic analysis.
    • The enhanced EOF and fluid transport facilitate efficient molecular separations.
    • Integrated PCR/CE on plastic chips demonstrates potential for portable genetic analysis systems.