Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved method for DNA extraction from paraffin sections.

Y G Man1, F Moinfar, G L Bratthauer

  • 1Department of Gynecologic and Breast Pathology, Armed Forces Institute of Pathology and American Registry of Pathology, Washington, DC 20306-6000, USA. man@afip.osd.mil

Pathology, Research and Practice
|September 25, 2001
PubMed
Summary

Optimized DNA extraction from paraffin sections is crucial for genetic analysis. New strategies ensure high-quality DNA yields, enabling consistent PCR product amplification for research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Independent validation of stromal uPA in ABCSG-08: Level 1b evidence for the prognostic value of uPA immunohistochemistry.

Breast (Edinburgh, Scotland)·2022
Same author

PAM-50 predicts local recurrence after breast cancer surgery in postmenopausal patients with ER+/HER2- disease: results from 1204 patients in the randomized ABCSG-8 trial.

The British journal of surgery·2021
Same author

A clinical validation study of MammaPrint in hormone receptor-positive breast cancer from the Austrian Breast and Colorectal Cancer Study Group 8 (ABCSG-8) biomarker cohort.

ESMO open·2021
Same author

Impact of Surgical Margins in Breast Cancer After Preoperative Systemic Chemotherapy on Local Recurrence and Survival.

Annals of surgical oncology·2019
Same author

Stromal coexpression of uPA/PAI-1 protein predicts poor disease outcome in endocrine-treated postmenopausal patients with receptor-positive early breast cancer.

Breast (Edinburgh, Scotland)·2019
Same author

32nd International Austrian Winter Symposium : Zell am See, the Netherlands. 20-23 January 2016.

EJNMMI research·2016

Area of Science:

  • Molecular Biology
  • Genetics
  • Histology

Background:

  • High-quality DNA extraction from paraffin-embedded tissues is essential for comparative genetic analyses.
  • Existing DNA extraction protocols often overlook critical factors like deparaffinization and cell-to-enzyme ratios, leading to extraction failures.

Purpose of the Study:

  • To develop and validate novel strategies for optimizing DNA extraction from paraffin-embedded tissue sections.
  • To address key factors limiting DNA yield and quality in previous methods.

Main Methods:

  • Implemented pre-incubation at 80°C before xylene treatment for efficient deparaffinization.
  • Introduced de-staining solutions for hematoxylin-stained sections and a micrometer-based method for cell counting to adjust enzyme volume.
  • Utilized magnifiers to monitor the digestion process.

Related Experiment Videos

Main Results:

  • Consistently obtained comparable DNA quality and quantity across multiple samples.
  • Achieved uniform Polymerase Chain Reaction (PCR) product amplification.
  • Demonstrated robustness despite variations in tissue embedding and processing.

Conclusions:

  • The developed strategies effectively overcome common challenges in DNA extraction from paraffin sections.
  • This optimized protocol ensures reliable DNA yields for downstream genetic applications, including PCR.
  • The method provides a practical approach for consistent results in molecular diagnostics and research.