Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New tools for oligonucleotide fingerprinting.

A Guerasimova1, L Nyarsik, I Girnus

  • 1Max-Planck-Institute for Molecular Genetics, Berlin, Germany. guer@molgen.mpg.de

Biotechniques
|September 26, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

John Raven, FRS, FRSE: a truly great innovator in plant physiology, photosynthesis and much more.

Photosynthesis research·2025
Same author

Sporadic bovine encephalopathy caused by Chlamydia pecorum secondary to bovine viral diarrhoea virus infection in calves in South Australia.

Australian veterinary journal·2023
Same author

Bedside open tracheostomy in COVID-19 patients - a safe and swift approach.

Medicina oral, patologia oral y cirugia bucal·2023
Same author

Final rinse water quality for flexible endoscopy to minimize the risk of post-endoscopic infection. Report from Healthcare Infection Society Working Party.

The Journal of hospital infection·2022
Same author

Measurement of ductions and fields of binocular single vision (BSV): orthoptic practice in the UK and Ireland.

Strabismus·2021
Same author

On the ecophysiology of the Clusiaceae in Trinidad: expression of CAM in Clusia minor L. during the transition from wet to dry season and characterization of three endemic species.

The New phytologist·2021

This study introduces fluorescently labeled peptide nucleic acid (PNA) probes for oligonucleotide fingerprinting, enhancing gene discovery in cDNA libraries. This method accelerates, simplifies, and reduces costs for high-throughput gene analysis.

Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • Oligonucleotide fingerprinting is a high-throughput method for gene discovery in cDNA libraries.
  • Current methods use radioactively labeled DNA oligonucleotides and sequential hybridizations.
  • Existing techniques face limitations in gridding density due to signal interference.

Purpose of the Study:

  • To develop an improved oligonucleotide fingerprinting technology.
  • To enhance the efficiency and reduce the cost of gene spectrum and abundance analysis.
  • To overcome limitations of radioactive labeling and hybridization methods.

Main Methods:

  • Utilized fluorescently labeled peptide nucleic acid (PNA) oligonucleotides as hybridization probes.
  • Employed a custom-designed, large-field, high-resolution laser scanner for data acquisition.

Related Experiment Videos

  • Implemented automated image analysis for efficient handling of hybridization images.

    • Main Results:

    • Significantly reduced signal interference, allowing for higher gridding density.
    • Achieved high sensitivity with fluorescence detection, enabling the use of nylon membranes.
    • Demonstrated substantial acceleration and simplification of hybridization data generation.

    Conclusions:

    • Fluorescent PNA oligonucleotides offer a superior alternative to radioactive probes for oligonucleotide fingerprinting.
    • The new system substantially improves hybridization data quality and reduces costs.
    • This enhanced method accelerates gene discovery and analysis in cDNA libraries.