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Low-power laser irradiation increases cyclic GMP synthesis in penile smooth muscle cells in vitro
N Kipshidze1, J R Petersen, J Vossoughi
1Lenox Hill Heart and Vascular Institute of New York, Cardiovascular Research Foundation, New York, New York 10021, USA. nkipshidze@lenoxhill.net
Objective:
The objective of the present study was to determine the effect of low-power laser irradiation (LPLI) on cyclic guanosine monophosphate (cGMP) produced by human corpus cavernosum smooth muscle cells (HCC SMC) in vitro.
Background Data:
Numerous reports suggest that LPLI is capable of affecting cellular processes in the absence of significant thermal effect
Methods:
HCC SMC cultures were irradiated with single-dose LPLI using a He-Ne continuous wave laser (632 nm) with different energy densities (0.52-2.1 J/cm2). Assessment of effect on cell viability was performed utilizing Alamar Blue assay. Effect of LPLI on cGMP production was studied by radioimmunoassay.
Results:
We observed that: (1) LPLI of HCC SMC results in a statistically significant increase of cGMP synthesis in culture and is dose dependent (maximal effect was observed with LPLI irradiance of 1.57 J/cm2. (2) There were no changes in Normalized Viability Index (NVI) immediately after and 24 h following laser irradiation.
Conclusion:
Our studies demonstrate that LPLI irradiation stimulates elevation of cGMP in vitro.
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