Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sodium/iodide symporter mRNA expression in cold thyroid nodules.

R Paschke1, S Neumann

  • 1III Medical Department, University of Leipzig, Germany. pasr@server3.medizin.uni-leipzig.de

Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [And] German Diabetes Association
|September 27, 2001
PubMed
Summary

Cold thyroid nodules (CTNs) show reduced sodium-iodide symporter (NIS) gene expression. Intra-individual comparisons reveal significantly lower NIS mRNA levels in 86% of CTNs, indicating NIS

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flow cytometric evaluation of monoclonal antibodies for cross-reactivity with feline leukocytes.

Frontiers in veterinary science·2026
Same author

Computed tomography can assess aortic valve stenosis by planimetry, enhances the continuity equation, reveals an anatomical ratio that mimics the dimensionless velocity index and demonstrates limitations in the use of calcification in stenosis severity assessment.

Clinical radiology·2026
Same author

Pain perception during baroreceptor unloading by lower body negative pressure.

European journal of pain (London, England)·2024
Same author

CT pulmonary angiography: optimising acute thoracic imaging by fixed-timing contrast medium delivery with a modified breathing instruction.

Clinical radiology·2023
Same author

Evaluation of Betulinic Acid Derivatives as PET Tracers for Hypoxia-Induced Carbonic Anhydrase IX (CA IX) Expression.

Advances in experimental medicine and biology·2022
Same author

Wind-borne dispersion of <i>Echinococcus multilocularis</i> eggs - a flight model.

Journal of helminthology·2022

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Thyroid Research

Background:

  • Clinical data suggest impaired thyroid hormone synthesis in cold thyroid nodules (CTNs).
  • The sodium-iodide symporter (NIS) is crucial for thyroid hormone synthesis.
  • Previous studies indicated variable reductions in NIS transcripts in CTNs.

Purpose of the Study:

  • To conduct an intra-individual comparison of NIS gene expression in CTNs.
  • To investigate the role of NIS in the pathogenesis of CTNs.

Main Methods:

  • Investigated 14 patients with one CTN.
  • Utilized RNase protection assay (RPA) for direct detection of NIS mRNA.
  • Compared NIS mRNA expression in nodules versus surrounding thyroid tissue.

Related Experiment Videos

Main Results:

  • Significantly reduced NIS expression was detected in 86% of CTNs compared to surrounding tissue.
  • NIS mRNA levels decreased by over 65% in 72% of the nodules studied.
  • Intra-individual analysis showed less variation in reduced NIS expression compared to previous studies.

Conclusions:

  • Reduced NIS expression is a common feature in CTNs.
  • Intra-individual comparison provides a more consistent view of NIS downregulation in CTNs.
  • Further research is needed to identify molecular factors influencing NIS expression in CTNs.