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Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene
J Yugueros1, A Temprano, M Sánchez
1Departamento de Sanidad Animal, Microbiología e Inmunología, Universidad de León, 24071 León, Spain.
Journal of Clinical Microbiology
|September 28, 2001
Abstract:
Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.