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Related Experiment Videos

An integrated fluorescence detection system in poly(dimethylsiloxane) for microfluidic applications.

M L Chabinyc1, D T Chiu, J C McDonald

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Analytical Chemistry
|September 29, 2001
PubMed
Summary

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This study presents an integrated fluorescence detection system using a microavalanche photodiode (microAPD) for microfluidic devices. The system demonstrates sensitive detection of analytes, suitable for bioanalytical applications.

Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Microfluidics

Background:

  • Microfluidic devices offer miniaturized platforms for various analytical applications.
  • Efficient and sensitive fluorescence detection is crucial for microfluidic systems.
  • Integrating photodetectors directly within microfluidic devices presents engineering challenges.

Purpose of the Study:

  • To develop and prototype an integrated fluorescence detection system for poly(dimethylsiloxane) (PDMS) microfluidic devices.
  • To utilize a microavalanche photodiode (microAPD) as the core photodetector.
  • To assess the system's performance for bioanalytical applications.

Main Methods:

  • Fabrication of PDMS microfluidic channels with an embedded optical fiber for excitation.

Related Experiment Videos

  • Integration of a prototype solid-state microAPD array within a PDMS slab.
  • Use of a polycarbonate filter to reduce scattered excitation light.
  • Operation of the microAPD in Geiger mode for fluorescence detection.

    • Main Results:

    • Achieved a detection limit of approximately 25 nM for fluorescein.
    • Demonstrated successful separation and detection of proteins and small molecules via capillary electrophoresis.
    • The microAPD's pixel size (30 microm) closely matched channel dimensions (50 microm).

    Conclusions:

    • The developed integrated system is suitable for sensitive fluorescence detection in microfluidic devices.
    • The prototype demonstrates potential for real-time bioanalytical applications.
    • The compact and integrated design eliminates the need for external transfer optics.