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Metabolic changes during platelet storage.

G Rock, A Figueredo

    Transfusion
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Storing platelets at 4°C preserves their function better than 22°C or 37°C. While lactate accumulation impacts platelet aggregation, it doesn't fully explain storage-related viability loss.

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    Area of Science:

    • Transfusion Medicine
    • Hematology
    • Biotechnology

    Background:

    • Platelet concentrates (PC) are crucial for treating bleeding disorders.
    • Optimal storage conditions for PC are essential to maintain platelet viability and function.
    • Metabolic changes during storage can impair platelet performance.

    Purpose of the Study:

    • To investigate the impact of different storage temperatures (4°C, 22°C, 37°C) on platelet metabolic parameters and function.
    • To determine the role of accumulated metabolites, particularly lactate, in platelet dysfunction during storage.
    • To assess the influence of oxygen and gas exchange on platelet viability markers.

    Main Methods:

    • Platelet concentrates were stored in plastic bags with continuous shaking at 4°C, 22°C, and 37°C for 72 hours.

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  • Metabolic parameters (pH, PO2, PCO2, lactate) and functional markers (aggregation, hypotonic shock response) were measured.
  • Experiments involved adding lactate to fresh PC and recombining aged and fresh components, including testing under degassed conditions.

    • Main Results:

    • Storage at 22°C led to decreased pH and PO2, and increased PCO2 and lactate, with a 70% decline in hypotonic shock response.
    • Storage at 4°C showed minimal metabolite accumulation and better aggregation, while 37°C resulted in significant metabolite buildup and loss of shock response.
    • Adding lactate to fresh PC inhibited aggregation but not hypotonic shock response. Recombining aged and fresh components under specific conditions surprisingly improved shock response and reduced lactate/CO2 accumulation.

    Conclusions:

    • Lactate accumulation is not solely responsible for the impaired platelet performance observed during storage at 22°C.
    • Storage temperature significantly affects platelet viability markers, with 4°C being superior for maintaining function.
    • Further investigation into gas exchange and other metabolic byproducts is needed to fully understand platelet storage lesions.