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Freezing and deglycerolizing sickle-trait red blood cells
Transfusion
|November 1, 1976
Summary
Sickle-trait red blood cells exhibit unique challenges during cryopreservation deglycerolization. Modifications were developed for cell washing systems, but satisfactory processing of sickle-trait cells remains difficult.
Area of Science:
- Biomedical Science
- Hematology
- Cryobiology
Background:
- Red blood cells (RBCs) are routinely cryopreserved for transfusion.
- Sickle-trait RBCs present unique challenges during post-thaw processing.
- Standard deglycerolization methods can cause damage to sickle-trait RBCs.
Purpose of the Study:
- To investigate the behavior of sickle-trait RBCs during cryopreservation.
- To develop and test modifications for deglycerolization of sickle-trait RBCs.
- To evaluate the efficacy of modified cell washing systems for sickle-trait RBCs.
Main Methods:
- Comparison of freezing and thawing responses between normal and sickle-trait RBCs.
- Analysis of RBC packing and hemolysis during centrifugal deglycerolization.
- Design and testing of modified deglycerolization protocols for Haemonetics and IBM systems.
Main Results:
- Sickle-trait RBCs behave similarly to normal cells during freezing and thawing.
- Deglycerolization by centrifugation causes packing and hemolysis in sickle-trait RBCs due to surface characteristic changes.
- Modified protocols were designed, but satisfactory deglycerolization of sickle-trait cells was not achieved.
Conclusions:
- Cryopreservation of sickle-trait RBCs requires specialized deglycerolization techniques.
- Surface changes in sickle-trait RBCs complicate processing in hyperosmotic solutions.
- Further research is needed to optimize deglycerolization for sickle-trait RBCs in automated systems.
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