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Expression of cytochrome P450 enzymes in the cervix. An immunohistochemical study
K.R. Patel1, S. Astley, D.J. Adams
1Academic Unit of Pathology, Departments of Clinical Medicine and Microbiology, University of Leeds, Department of Genitourinary Medicine and Gynaecological Oncology Unit, Clarendon Wing, United Leeds Teaching Hospitals NHS Trust, Leeds, UK.
Abstract:
The expression of cytochrome P450 isoenzymes (subfamilies) CYP1A, CYP2B, CYP2C, CYP3A and CYP4A in the histologically normal cervix was explored using a panel of polyclonal antibodies. There was variation in the intensity of immunohistochemical reaction between the isoenzymes and between the various components of the cervix. Half the subjects tested were smokers and had increased urinary cotinine levels. Statistical analysis revealed no significant differences between smokers and nonsmokers in the expression of these isoenzymes. The implications of these observations in relation to cervical carcinogenesis are discussed.