Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Wnts, signaling and sulfates.

S S Blair1

  • 1Department of Zoology, University of Wisconsin, 250 N. Mills St., Madison, WI 53706, USA. ssblair@facstaff.wisc.edu

Science'S STKE : Signal Transduction Knowledge Environment
|October 2, 2001
PubMed
Summary

Sulfated glycosaminoglycans are key regulators of developmental signaling pathways. A novel sulfatase, Qsulf1, influences vertebrate cell sensitivity to Wnt signaling by altering glycosaminoglycan sulfation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell lineage: compartments and Capricious.

Current biology : CB·2001
Same author

Notch signaling: Fringe really is a glycosyltransferase.

Current biology : CB·2000
Same author

Crossveinless 2 contains cysteine-rich domains and is required for high levels of BMP-like activity during the formation of the cross veins in Drosophila.

Development (Cambridge, England)·2000
Same author

Dorsoventral lineage restriction in wing imaginal discs requires Notch.

Nature·1999
Same author

Eye development: Notch lends a handedness.

Current biology : CB·1999
Same author

Limb development: marginal fringe benefits.

Current biology : CB·1998

Area of Science:

  • Developmental biology
  • Cell signaling
  • Biochemistry

Background:

  • Pattern formation during development relies on intricate signaling pathways.
  • Sulfated glycosaminoglycans (GAGs) are known to modulate several key developmental signals.
  • The precise mechanisms by which GAGs influence these pathways are not fully understood.

Purpose of the Study:

  • To investigate the role of sulfated glycosaminoglycans in developmental signaling.
  • To identify novel regulators of GAG-mediated signaling.
  • To elucidate the function of the secreted sulfatase Qsulf1 in vertebrate development.

Main Methods:

  • Analysis of signaling pathway activity in response to GAG modification.
  • Biochemical assays to assess GAG sulfation levels.
  • Functional studies using Qsulf1 in vertebrate cell models.

Main Results:

  • Sulfated GAGs were shown to impact Wnt-Wingless, Fibroblast growth factor, Hedgehog, and Bone morphogenetic protein-4 signaling pathways.
  • A newly identified secreted sulfatase, Qsulf1, was found to regulate vertebrate cell sensitivity to Wnt signaling.
  • Qsulf1 activity appears to be linked to the modification of GAG sulfation patterns.

Conclusions:

  • Sulfated GAGs play a critical role in orchestrating multiple developmental signaling pathways.
  • Qsulf1 emerges as a novel regulator of Wnt signaling sensitivity.
  • Modulation of GAG sulfation by enzymes like Qsulf1 is a key mechanism in developmental pattern formation.

Related Experiment Videos