A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase
H Uchida1, H Morinaga, T Misaki
1Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan. uchida@acbio.acbio.fukui-u.ac.jp
Nucleosides, Nucleotides & Nucleic Acids
|October 3, 2001
Abstract:
By affinity chromatography with Sepharose coupled to 2'-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated.


