Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Guiding epitaxial crystallization of amorphous solids at the nanoscale: Interfaces, stress, and precrystalline order.

The Journal of chemical physics·2022

Same author

Non-Invasive MRI of Blood-Cerebrospinal Fluid Barrier Function.

Nature communications·2020

Same author

Single-Molecule FRET to Measure Conformational Dynamics of DNA Mismatch Repair Proteins.

Methods in enzymology·2016

Same author

Two-party secret key distribution via a modified quantum secret sharing protocol.

Optics express·2015

Same author

A short-pulse X-ray beamline for spectroscopy and scattering.

Journal of synchrotron radiation·2014

Same author

Extraordinary optical transmission of multimode quantum correlations via localized surface plasmons.

Physical review letters·2014

Search research articles

Related Experiment Video

Updated: Jul 22, 2026

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Published on: May 9, 2021

Comment on "Mie scattering from a sonoluminescing bubble with high spatial and temporal resolution".

K R Weninger¹, P G Evans, S J Putterman

¹Physics Department, University of California, Los Angeles, California 90095, USA.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics

|October 3, 2001

Summary

Sonoluminescence (SL) research disputes findings on bubble wall velocity measurements. Discrepancies in light scattering data suggest potential artifacts in prior experimental arrangements, impacting velocity calculations.

More Related Videos

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature

Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature

Published on: November 14, 2025

Related Experiment Videos

Last Updated: Jul 22, 2026

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Published on: May 9, 2021

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature

Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature

Published on: November 14, 2025

Area of Science:

Physics
Acoustics
Optics

Background:

Sonoluminescence (SL) is a phenomenon linked to the dynamics of collapsing bubbles.
Accurate measurement of bubble radius R(t) over time is crucial for understanding SL.
Light scattering is a proposed method for measuring bubble radius and velocity.

Purpose of the Study:

To address discrepancies in light scattering measurements of sonoluminescent bubble dynamics.
To refute claims by Gompf and Pecha regarding the timing of scattered light minima relative to SL.
To analyze potential artifacts in experimental setups used for measuring bubble wall velocity.

Main Methods:

Analysis of Mie scattering data from collapsing bubbles.
Critical evaluation of experimental arrangements for sonoluminescence studies.
Theoretical discussion of light scattering phenomena in relation to bubble dynamics.

Main Results:

Disagreement with the assertion that scattered light minima precede SL by 700 ps.
Identification of potential experimental artifacts leading to overestimated bubble wall velocities.
Rebuttal of criticisms concerning Mie scattering experiments on sonoluminescence.

Conclusions:

The timing of scattered light minima is not a reliable indicator preceding SL as previously stated.
Experimental artifacts can significantly affect the accuracy of bubble wall velocity measurements.
Further refinement of experimental techniques is necessary for precise sonoluminescence research.