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Related Experiment Videos

Pseudomonas fluorescens as a potential pathogen: adherence to nerve cells.

L Picot1, S M Abdelmoula, A Merieau

  • 1Laboratory of Cold Microbiology, UPRES2123, University of Rouen, 55, rue Saint Germain, 27000 Evreux, France.

Microbes and Infection
|October 3, 2001
PubMed
Summary

Pseudomonas fluorescens binds to nerve cells, impacting their function and potentially causing apoptosis. This suggests Pseudomonas fluorescens may act as a pathogen, similar to Pseudomonas aeruginosa.

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Area of Science:

  • Microbiology
  • Neuroscience
  • Pathogen Research

Background:

  • Pseudomonas fluorescens is a psychrotrophic bacterium closely related to the opportunistic pathogen Pseudomonas aeruginosa.
  • Understanding the infectious potential of P. fluorescens is crucial due to its relation to P. aeruginosa.

Purpose of the Study:

  • To investigate the binding activity and potential pathogenicity of Pseudomonas fluorescens on neuronal and glial cells.
  • To compare the binding of P. fluorescens to that of P. aeruginosa.

Main Methods:

  • Primary cultures of rat neonate cortical neurons, glial cells, adrenal paraneurons, and NG108-15 neuroblastoma cells were used.
  • Cells were incubated with P. fluorescens MF37 and P. aeruginosa PAO1 at concentrations of 10(6) and 10(8) CFU/mL.
  • Binding assays and fluorescent microscopy with Hoechst 33258 were employed to assess cell interaction and apoptosis.

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Main Results:

  • P. fluorescens exhibited high binding activity on neurons, comparable to P. aeruginosa.
  • Significant adherence was observed on glial cells and adrenal paraneurons, but not on neuroblastoma cells.
  • P. fluorescens induced DNA changes indicative of apoptosis in neurons and glial cells, including cytoplasmic leakage and nuclear morphological alterations.

Conclusions:

  • Pseudomonas fluorescens demonstrates significant binding to nerve cells and affects their physiology.
  • The observed effects on neurons and glial cells suggest P. fluorescens may possess pathogenic capabilities.
  • These findings align with recent clinical observations, supporting the potential of P. fluorescens as a pathogen.