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The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS

T Sugiyama1, N Koide, D Chakravortty

  • 1Department of Microbiology and Immunology, and Division of Bacterial Toxin, Research Center for Infectious Disease, Aichi Medical University, Nagakute, Aichi, Japan. sugiyama@aichi-med-u.ac.jp

Journal of Endotoxin Research
|October 3, 2001
PubMed

Insights

Mouse B-1 cells express CD14, a molecule crucial for their response to lipopolysaccharide (LPS). This suggests B-1 cells utilize membrane-bound CD14 to detect and react to LPS.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • B-1 cells are a distinct subset of lymphocytes with unique immune functions.
  • Lipopolysaccharide (LPS) is a potent activator of the innate immune system, primarily recognized by Toll-like receptor 4 (TLR4).
  • CD14 is a pattern recognition receptor involved in LPS sensing.

Purpose of the Study:

  • To investigate the surface expression of CD14 on mouse B-1 cells.
  • To determine the role of CD14 in the response of B-1 cells to LPS.

Main Methods:

  • Utilized the murine TH2.52 B-1 cell line and primary peritoneal B-1 cells.
  • Assessed CD14 surface expression via flow cytometry.
  • Stimulated cells with LPS and measured activation of nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) pathways.
  • Investigated the effect of anti-CD14 antibody on LPS-induced signaling.

Main Results:

  • Both the TH2.52 B-1 cell line and primary peritoneal CD5+ B-1 cells expressed membrane-bound CD14.
  • LPS stimulation of CD14-expressing TH2.52 cells induced NF-κB and MAPK activation.
  • Anti-CD14 antibody significantly inhibited LPS-induced NF-κB and MAPK activation.

Conclusions:

  • Mouse B-1 cells express CD14 on their surface.
  • Membrane-bound CD14 plays a significant role in mediating the activation of B-1 cells by LPS.
  • These findings suggest a novel mechanism for B-1 cell recognition of LPS through CD14.

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