Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reconstitution of a functional core polycomb repressive complex.

N J Francis1, A J Saurin, Z Shao

  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

Molecular Cell
|October 5, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Datopotamab deruxtecan in patients with untreated, advanced triple-negative breast cancer (TROPION-Breast02): a randomised, open-label, international, phase III trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

Daytime dysfunction and SUDEP risk: Exploring the role of sleep and heart rate variability in epilepsy.

Neurologia·2026
Same author

Overall survival with abemaciclib in early breast cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Correction to: IL-4 administration exerts preventive effects via suppression of underlying inflammation and TNF-α-induced apoptosis in steroid-induced osteonecrosis.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2025
Same author

Mandibular reconstruction with a custom-made CAD/CAM porous titanium prosthesis in a 10-year-old patient and the long-term prognosis.

International journal of oral and maxillofacial surgery·2025
Same author

Intelligent Diagnosis of Cervical Lymph Node Metastasis Using a CNN Model.

Journal of dental research·2025
Same journal

Thyroid cancer-associated EZH1 Q571R mutation drives chromatin compaction and H3K27me3 invasion into active chromatin.

Molecular cell·2026
Same journal

Genome-wide rotational and translational phasing of nucleosomes with human transcription factors.

Molecular cell·2026
Same journal

Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation.

Molecular cell·2026
Same journal

Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.

Molecular cell·2026
Same journal

A bacterial reverse transcriptase: Protein-templated DNA synthesis fuels antiviral immunity.

Molecular cell·2026
Same journal

Tweezing apart ribosome heterogeneity.

Molecular cell·2026
See all related articles

Polycomb repressive complex 1 (PRC1) proteins establish gene repression by inhibiting chromatin remodeling. A core PRC1 complex and PSC protein were found to block SWI/SNF activity, maintaining normal chromatin structure.

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Chromatin Biology

Background:

  • Polycomb group (PcG) and trithorax group (trxG) proteins are crucial for maintaining gene expression patterns during development.
  • PcG proteins are known to establish repressive chromatin structures, but the precise mechanisms remain unclear.
  • Polycomb repressive complex 1 (PRC1) is implicated in PcG-mediated repression and inhibits chromatin remodeling by SWI/SNF complexes.

Purpose of the Study:

  • To define the functional core of Polycomb repressive complex 1 (PRC1).
  • To investigate the mechanism by which PRC1 inhibits chromatin remodeling by SWI/SNF complexes.

Main Methods:

  • Reconstitution of a stable PRC1 complex using four recombinant PcG proteins.
  • Biochemical assays to assess the effect of PRC1 and its subunits on chromatin remodeling by SWI/SNF complexes.

Related Experiment Videos

  • Analysis of nucleosome organization and DNA accessibility in the presence of PRC1.

    • Main Results:

    • A stable functional core of PRC1 was reconstituted from four recombinant PcG proteins.
    • The PSC subunit of PRC1 was found to inhibit chromatin remodeling independently.
    • PRC1 creates a chromatin structure with normal nucleosome organization that is accessible to nucleases but excludes hSWI/SNF.

    Conclusions:

    • The study defines a functional core of PRC1, providing mechanistic insights into PcG-mediated gene repression.
    • PRC1 directly inhibits the activity of SWI/SNF chromatin remodeling complexes.
    • These findings contribute to understanding how PcG proteins establish and maintain repressive chromatin states during development.