Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A model for the gamma delta resolvase synaptic complex.

G J Sarkis1, L L Murley, A E Leschziner

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

Molecular Cell
|October 5, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.

Nature·2020
Same author

Tug-of-war in motor protein ensembles revealed with a programmable DNA origami scaffold.

Science (New York, N.Y.)·2012
Same author

Structural basis for microtubule binding and release by dynein.

Science (New York, N.Y.)·2012
Same author

The basis of asymmetry in IS2 transposition.

Molecular microbiology·2001
Same author

Contacts between the 5' nuclease of DNA polymerase I and its DNA substrate.

The Journal of biological chemistry·2001
Same author

In vitro transposition system for efficient generation of random mutants of Campylobacter jejuni.

Journal of bacteriology·2001

This study proposes a new structural model for the serine recombinase gamma delta resolvase synaptic complex, revealing key interactions and suggesting a significant rearrangement for DNA recombination.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Genetics

Background:

  • Serine recombinase gamma delta resolvase mediates site-specific DNA recombination.
  • This process involves a complex synaptic structure with 12 resolvase subunits and two DNA recognition sites (res sites).

Purpose of the Study:

  • To present an alternative structural model for the gamma delta resolvase synaptic complex.
  • To elucidate the subunit interactions and their role in DNA recombination.

Main Methods:

  • Structural modeling of the synaptic complex.
  • Analysis of mutant resolvase interactions at the subunit interface.
  • Investigating the spatial arrangement of DNA crossover sites.

Main Results:

Related Experiment Videos

  • A novel structural model for the synaptic complex is proposed.
  • Resolvase subunits engage in equivalent interactions, including a newly identified synaptic interaction.
  • Mutational analysis supports the proposed synaptic interaction and its role in synapsis.

Conclusions:

  • The proposed model suggests crossover sites are distant, requiring significant rearrangement of subunits or domains for recombination.
  • This provides new insights into the mechanism of serine recombinase-mediated DNA recombination.