Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene-trap mutagenesis: past, present and beyond.

W L Stanford1, J B Cohn, S P Cordes

  • 1Programme in Development and Fetal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Room 983, Toronto, Ontario, Canada M5G 1X5. stanford@mshri.on.ca

Nature Reviews. Genetics
|October 5, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating dose-limiting toxicities of MDM2 inhibitors in patients with solid organ and hematologic malignancies: A systematic review of the literature.

Leukemia research·2019
Same author

Engineering of hyaline cartilage with a calcified zone using bone marrow stromal cells.

Osteoarthritis and cartilage·2015
Same author

Critical components of the pluripotency network are targets for the p300/CBP interacting protein (p/CIP) in embryonic stem cells.

Stem cells (Dayton, Ohio)·2013
Same author

Forward genetic screen of mouse reveals dominant missense mutation in the P/Q-type voltage-dependent calcium channel, CACNA1A.

Genes, brain, and behavior·2007
Same author

Molecular genetics of the early development of hindbrain serotonergic neurons.

Clinical genetics·2005
Same author

Molecular genetics of cranial nerve development in mouse.

Nature reviews. Neuroscience·2001
Same journal

Genetic origins and constraints of evolutionary innovation.

Nature reviews. Genetics·2026
Same journal

Single-cell four-omics with CHARM.

Nature reviews. Genetics·2026
Same journal

Molecular integration of seasonal temperature signals in flowering time control.

Nature reviews. Genetics·2026
Same journal

RBPscan measures protein-RNA interactions in living cells.

Nature reviews. Genetics·2026
Same journal

Revisiting retinal and macular degeneration in the genomics era.

Nature reviews. Genetics·2026
Same journal

How evolution builds three morphs from one genome.

Nature reviews. Genetics·2026
See all related articles

Gene-trap mutagenesis creates loss-of-function mutations to study gene expression. This genomic technology advances research by providing over 8,000 mutagenized embryonic stem cell lines for public use.

Area of Science:

  • Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Limited functional and expression data exists for a majority of sequenced human genes.
  • Gene-trap mutagenesis offers a method to identify gene function and expression patterns.
  • Current large-scale efforts are creating a valuable public resource of mutagenized mouse embryonic stem cells.

Purpose of the Study:

  • To evaluate recent advancements in gene-trap mutagenesis technology.
  • To identify remaining technical challenges in the field.
  • To highlight the utility of the growing public resource of mutagenized ES cells.

Main Methods:

  • Random gene-trap mutagenesis in mouse embryonic stem cells.
  • Development and application of novel gene-trap vectors.

Related Experiment Videos

  • Large-scale screening and characterization of mutagenized cell lines.

    • Main Results:

    • Generation of a public resource exceeding 8,000 mutagenized ES-cell lines.
    • Demonstration of gene-trap mutagenesis as an effective tool for discovering gene function.
    • Identification of numerous novel gene expression patterns.

    Conclusions:

    • Gene-trap mutagenesis is a powerful genomic technology for functional gene discovery.
    • The expanding public resource of mutagenized ES cells significantly aids research.
    • Continued technological refinement is necessary to overcome existing hurdles in gene-trap screening.