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Related Experiment Videos

Gene-trap mutagenesis: past, present and beyond.

W L Stanford1, J B Cohn, S P Cordes

  • 1Programme in Development and Fetal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Room 983, Toronto, Ontario, Canada M5G 1X5. stanford@mshri.on.ca

Nature Reviews. Genetics
|October 5, 2001
PubMed
Summary

Gene-trap mutagenesis creates loss-of-function mutations to study gene expression. This genomic technology advances research by providing over 8,000 mutagenized embryonic stem cell lines for public use.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Limited functional and expression data exists for a majority of sequenced human genes.
  • Gene-trap mutagenesis offers a method to identify gene function and expression patterns.
  • Current large-scale efforts are creating a valuable public resource of mutagenized mouse embryonic stem cells.

Purpose of the Study:

  • To evaluate recent advancements in gene-trap mutagenesis technology.
  • To identify remaining technical challenges in the field.
  • To highlight the utility of the growing public resource of mutagenized ES cells.

Main Methods:

  • Random gene-trap mutagenesis in mouse embryonic stem cells.
  • Development and application of novel gene-trap vectors.

Related Experiment Videos

  • Large-scale screening and characterization of mutagenized cell lines.

    • Main Results:

    • Generation of a public resource exceeding 8,000 mutagenized ES-cell lines.
    • Demonstration of gene-trap mutagenesis as an effective tool for discovering gene function.
    • Identification of numerous novel gene expression patterns.

    Conclusions:

    • Gene-trap mutagenesis is a powerful genomic technology for functional gene discovery.
    • The expanding public resource of mutagenized ES cells significantly aids research.
    • Continued technological refinement is necessary to overcome existing hurdles in gene-trap screening.